Friday, October 31, 2014

Thank A Microbe Exhibition Day (28/10/14)

My Reflection

Our group poster !

Our group video on the introduction of Clostridium novyi-NT.


Another Youtube videos with more detailed explanation

Introduction

"Thank a Microbe" is a project planned by Dr. Wan for us to let us have appreciation on those beneficial microbes that have done positive impacts on human and environment. The microbe world is not merely harmful to us, instead most of the microbes are actually closely related to us and we cannot live without them.

Preparation

Finding a suitable microbe to thank of is actually quite challenging to our group because we want to find something special and significant. At first we planned to thank some other microbes, but due to the insufficient information and not-so-significant-information found, at last we reject some (bacteriophage, Ganoderma sp., Penicillum) and finally choose Clostridium novyi-NT as our main character.

Exhibition Eve

I actually thought that the exhibition would be in Friday but in fact it's the second day right after I realized it. And on that day, we will be having another presentation as well (although in the end it turned out to be postponed), but having the progress stuck and unable to do anything (like wifi couldn't work at all) make me feel stressed. So at that moment, I actually felt like going to have a big nap and recharge myself  before going start all these things. I felt very sorry and would like to apologize to those who felt uncomfortable seeing my "uncomfortable", especially my groupmates. I just need some rest ~ o.O

Exhibition Day

It was chaos at first because Aurasma was not working. I found that maybe it's because the triggered image I uploaded was from computer, which was clearer from the real poster, so maybe the camera couldn't detect it. Despite that, the whole exhibition session was really an awesome one. I was enjoying myself but the session was just too short for me !

There're some lessons learnt throughout the session. Although we couldn't answer some of the questions, but I did try to answer, even though it's wrong one. I found that in times, if I really do not know about the concept or the correct information, I should just admit that I do not know and do some research on the topic later on. This is because in science-related field, it will be very misleading if we are giving the wrong information. Of course, as Dr. Wan said, this session did make us realize that our knowledge are limited and there are a lot more need to be explored.

I was glad that I chose to continue explaining my poster even though the judges were gone. I saw many inquisitive faces which amazed at the result of this founding - using bacteria to fight tumor. I felt glad of the scientists, for what they found really helped the world. Moreover, I felt glad that I found the information and was able to share it out. More people should know about the new technology we are having now which makes our life better than we can imagine.

Plus, while I was still explaining, I came across with a master student who studied virology and cancer. He gave me lots of information which makes me realized that there are people who really knows about all these stuffs because they really study it in detailed. They're unlike us, who just read up some information and bluffed on it. From him, I knew that correct style of people in scientific field or even just science students.

Lastly from the exhibition, I saw the efforts done by my coursemates. They were really passionate at running the exhibition by preparing suitable props while we our group just did the poster only. Thanks to them too and Dr. Wan because they've prepared a lots of food for us, that was one of the greatest thing that made my day!

Overall

All in all, the exhibition day was a great one ! Hope we will have opportunities to hold another one in the future !

Friday, October 17, 2014

Lesson 10 (14/10/14)

Date : 14/10/14
Topic : Protists (Algae)

Activities
1. BYON (Bring Your Own Notes)

Notes

Name of microbes


Algae can be separated into seven major divisions.
  • Chlorophyta (Green algae) includes single-celled Spyrogyra with filament and flat-leaf mass of cells called thallus (Ulva lactuca).
  • Phaeophyta (Brown algae) includes familiar seaweeds found on rocky shores
  • Rhodophyta (Red algae) includes those found in warmer seas and several species of economic importaance. 
  • Chrysophyta (Golden-brown algae) includes diatoms such as bacillariophyta.
  • Xanthophyta (Yellow-green algae)
  • Pyrrophyta (Dinoflagellata)
  • Euglenophyta
divisions of algae

Rhodophyta (red algae) is a group of multicellular aquatiic algae with highly varied organisation of plant body. They have cellulose cell wall or agar and store glucose polymer. They can be found atached to rocky stones. Vegetative reproduction occurs by fragmentation. Asexual reproduction involves formation of different types of spores like monospores, neutral spores, corpospores and tetraspores.  Sexual reproduction occurs by oogamy carried out by antheridia or spermatangia and carpogonia. Life cycle involves alternate generation of haploid gametophytic generation and diploid sporophytic generation. They contain chloropyll a, chlorophyll d, phycocyaninphycoerythrin,
multicellular members of red algae family

Phaeophyta (brown algae // seaweed) is a much-advance group of marcoscopic algae found mostly in the sea. They have cellulose cell wall or algin and store carbohydrates. The plant body may be filamentous, hold fast and stemlike stipe, or leaf-like blades. Vegetative reproduction occurs by fragmentation. Asexual reproduction involves formation of motile zoospores and non-motile spores (monospores and tetraspores). Sexual reproduction may be isogamy (fusion of similar gametes), anisiogamy (fusion of dissimilar gametes) or oogamy (a form of anisogamy in which non-motile female gamate is larger than male gamate). It contains fucoxanthin, chlorophyll a, chlorophyll c and xanthophylls.
memebers of brown algae family

Chlorophyta is a diverse group of algae and mostly found in fresh water. It has cellulose cell wall and store glucose polymer. It contains chlorophyll a, chlorophyll b, carotenes and xanthophylls. Vegetative reproduction occurs by cell division, fragmentation and stolon formation (creeping horizontal plant stem or runner that can form new plants). Asexual reproduction occurs by fission and formation of zoospores or aplanospores. Sexual reproduction can be isogamous, anisogamous, or oogamous. Types of plant body:
  • Unicellular and motile = Chlamydomonas
  • Colonial and motile = Volvox
  • Colonial and non-motile = Hydrodictyon
  • Filamentous = Oedogonium and Cladophora
organisms of green algae

Chrysophyta contains golden-brown algae, such as diatoms. They contain carotenoids, chlorophyll a and chlorophyll c. Diatoms are algae which have a variety of intricate shapes and contain silicon in their cell walls. Diatoms are unlike other algae under Chrysophyta, they are either nonmobile or glide slowly along surfaces. They store material in oil. They are important in global carbon cycling.



Pyrrophyta or dinoflagellates are unicellular planktons with cellulose cell wall. They have two flagella in perpendicular opposing grooves. They can produce neurotoxins and cause red tides. They contain chlorophyll a and chlorophyll c.
Dinoflagellates

Eugleonophyta or euglenoid algae contain green or colorless flagellates. Some consider euglenoid algae as protozoans because some do not contain chloroplasts and able to feed on organic matter and other organisms.They store food in glucose polymer. They have no cell wall but have pellicle or rigid plasma membrane. No sexual reproduction occurs. They contain chlorophyll a, chlorophyll b, carotene. However, they can spontaneously lose chlorophyll.

Euglena is a typical euglenoid. It has a single large flagellum and several chloroplasts.

Coralline algae are red algae under order Corallinales. It is part of the coral reefs.

Clamydomonas is a typical green alga with two flagella. Each Chlamydomonas cell contains a single, large chloroplast which fills up most of the cell. It also contains an eyespot which is a light-sensitive organelle that helps Chlamydomonas to swim towards light.

Terms in microbiology

Osmotrophy is the uptake of dissolved organic compounds by osmosis for nutrition.

Hydrogenosome is a membrane-enclosed organelle of some anaerobic ciliates, trichomonads and fungi.

Trichomonad is a parasitic protozoan with four to six flagella and an undulating membrane. It infests the urogenital or digestive system.

Syngamy is the fusion of two cells or fusion of their nuclei, in reproduction ; Autogamy is the process of self-fertilization, especially the self pollination of flower.

Thallus is an undifferentiated plant body.

Aplanospores are non-motile spores ; Zoospores are flagellated motile spores.

Antheridia and oogonia are gamates of algae, mosses, ferns, fungi, and other non-flowering plants.

Random facts
  • Cytoplasm of protists sometimes divided into outer gelatinous ectoplasm and inner fluid-like endoplasm.
  • Pellicle provides support.
  • Algae can be unicellular or colonial, microscopic or marcoscopic, can have simple or complex filaments. Habitat of algae can be aquatic habitat, moist soils, dry soils or acidic habitat, Algae may have no cell wall or have cell wall strengthened with calcium carbonate, chitin or silica. The cell wall mostly has modified cellulose fibrils which have added polysaccharides such as pectic, xylan and mannans. Algae can be found as in terrestrial (moist rocks, trees, soil, or as endosymbionts - as in protozoa, worms, corals and fungi), planktonic, benthic (bottom of ocean or lakes) or neustonic (surace layer of water) form.
  • Algae can be used to make biofuels (Biodiesel, Bioethanol,  Biobutanol), biomass and produce vegetable oils, supplements and hydrogen.
  • Algae can control pollution and is used in wastewater treatment facilities. Algae can capture fertilizers in runoff from farms. When subsequently harvested, the enriched algae can be used as fertilizers. Algae bioreactors are used to reduce CO2 emission
  • Alginic acid of phaeophyta can be used for thickening, stabilizing and emusifying purposes. For example, in making ice cream and dental impressions.
  • Consequences of algae bloom include disrupting higher links of food web and producing neurotoxin. Neurotoxin produced by algae accumulated in fishes or shellfishes. Example of algae bloom is red tide caused by dinoflagellates, the brevetoxins produced cause respiratory irritation in human. Some algae bloom will consume itself and cause bacterial bloom.


Muddiest point
-

My own exploration
Reflection on this topic

The topic of protists is quite interesting because there is no distinct characteristics to distinguish each order. The taxonomy and grouping of protists are changing from time to time when new discovery is found. This makes me realize that there is still much discoveries have to be done on microorganisms. We can actually discover them ourselves even though many research has been done before us.

However, it takes me quite some time to understand the topic of algae. For this topic, I plan to make a table to classify the characteristics of each order under algae.


Sunday, October 12, 2014

Lesson 9 (10/10/14)

Date : 10/10/14
Topic : Test 1 and protozoans

Activities
1. Test
2. Group discussion and presentation on the best method to study the topic of protozoans

Notes

Name of microbes

Protists consist of "animal-like" protozoa, "plant-like" protophyta (mostly unicellular algae), "fungus-like" slime molds and water molds. Many protists (like algae) are vital primary producers in ecosystems (plankton). Some protists (like Trypanosoma brucei and Plasmodium) cause diseases. Another definition of protists is that protists are essentially unicellular eukaryotes that either exist as independent cells or undifferentiated if in colony. Since protists as a whole are paraphyletic, some will eventually split up or abandon the kingdom.

Protozoa refer to heterotrophic species of protists that do not form filaments. They are larger than prokaryotes and is eukaryotic. They have no chlorophyll. They are motile and lack of cell wall and fruiting body. Protozoa are divided into four major groups : ciliates (Ciliophora), flagellates (Archaezoa), heliozoans, amoebas (Rhizopoda).

Slime molds (Myxostelida) are organisms that use spores to reproduce and have fruiting bodies. They normally exist as single-celled organisms, but will congregate and start moving as a single body when food is in short supply. They feed on microorganisms that live in any type of dead plant material, such as in soil, lawns, on forest floor and deciduous logs. Slime mold  is not a real fungus even though it's a mold. It is classified under protista because fungus absorbs food from the environment while slime mold is like a plasmodium (a giant amoeba) that moves around slowly in decaying organic matter and eat up the bacteria.

Entodiniomorphids are group of ciliates that occur in the stomach of cattle, sheep and goat. For example, Diplodinium dendatum in cattle.

Holotrich or holotrichous are organisms that have cilia of uniform length which distributed evenly over the surface of the body.

Trypanosoma gambiense is a genus of kinetoplastids (unicellular parasitic flagellate protozoa).

Joenia is a protozoan (animal-like unicellular protozoan) that is motile. It is restricted to moist or aquatic habitat and contains non-filamentous structures. It is also a gut flagellate that can be found from the termite Kalotermes flavicollis. It has extended axostyle, attached spirachetes on body surface and tuft of flagella on the anterior that looks like horses mane.
(E.g. Joenia annectens)
This item is an imageImage of Joenia annectensImage of Joenia annectensWEB Image_3077 Joenia having a good hair day

Devescovina is a flagellates with an elongated body
Image of DevescovinaImage of Devescovina

Dinoflagellate is a single-celled organism with two flagella, occurring in large numbers in marine plankton and also found in fresh water. Some produce toxins that can accumulate in shellfish, resulting in poisoning when eaten. Some are photosynthetic, but most are mixotropic that carry out photosynthesis and ingest prey. Dinoflagellate is also the most common source of bioluminescence.


















Euglenoid is a flagellated unicellular organism of a group that comprises euglena and its relatives. They are mixotrophic, most are photosynthetic but some carry out phagocytosis too. They are commonly found in freshwater, especially with high organic materials. They are endosymbiotic (live within body or cells of another organism). Euglenoids do not have cell wall but possess pellicle and flagella. They have euglenoid movement, which moves by contraction and expansion of body. Some glide rather than making euglenoid movement.


Amoeba proteus is a large unicellular, colorless or transparent protozoan. It prefers to habitat clean ponds of highly oxygenated fresh water. It is adverse to light, thus will take cover under things that provide shade such as lilly pads.


Entameoba histolytica is an anaerobic parasitic protozoan that infects the digestive tract of predominantly humans and other primates.


Balantidium coli is a parasitic ciliate protozoan that cause Balantidiasis. It is the only protozoan ciliate to infect humans. It is also the largest protozoan parasite found in humans. Balantidium coli has two stage of life, trophozoite (active, motile feeding stage) and cyst. The cysts they formed will contaminate food or water and grow into trophozoite if survive in small intestine. Trophozoites live in cecum and colon of large intestines and may encyst once faeces dry up. Symptoms of balantidiasis include diarrhea or constipation, weight loss and dysentery.
Balantidium coli life cycle

Plasmodium vivax is a parasitic protozoa found on Anophele mosquito vectors and is human pathogen that causes malaria. Symptoms of malaria include fever, chills, weakness, headache, vomiting, diarrhea, anemia, pulmonary and renal dysfunction and neurologic changes.


Toxoplasma is a parasitic spore-forming protozoan that can cause toxoplasmosis in human.

Terms in microbiology

Normal flora are bacteria which are found in or on our bodies on a semi-permanent basis without causing disease.

Schizogony is a type of asexual reproduction by multiple fission. It is found in some protozoa, especially parasitic sporozoans.

Substratum is an underlying layer of rock or soil beneath the surface of the ground.

Lorica is a rigid case or shell of some rotifers (a minute multicellular aquatic animal of phylum Rotifera) and protozoans.

Encystment is a process preceding reproduction by budding, fission and spore formation. For example, the amoeba becomes circular, dehydrates and secretes a protective cyst membrane.

Pinocytosis is the ingestion of liquid into a cell by budding off small vesicles from the cell membrane.

Gullet is the passage by which food passes from the mouth to the stomach // the oesophagus.

Cytostome or cell mouth is part of a cell specialized for phagocytosis, usually in the form of a microtubule-supported funnel or groove. Food directed into cytostome is sealed into vacuoles.

African sleeping sickness is a parasitic disease of humans and other animals caused by protozoa such as Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense. Symptoms include chancre (red sore), fever, sever headaches, irritability, extreme fatigue, swollen lymph nodes, skin rash, aching muscles and joints. Those symptoms affect the quality of sleeping.

Dysentery is the infection of the intestines resulting in severe diarrhea with the presence of blood or mucus in faeces.

Particulate material is a mixture of solid particles and liquid droplets found in air. Some are large or dark enough to be seen with naked eyes, such as dust, dirt, soot or smoke.

Balantidiasis is a protozoan infection caused by infection with Balantidium coli.

Pellicle is a think skin, cuticle, membrane or film. The cilia are usually arranged on the pellicle meanwhile the cilia can also fuse togehter near the cytostome.

Obligate parasite cannot complete its life cycle without exploiting a suitable host, meaning it will fail to reproduce if it cannot obtain a host.

Endosymbiont lives within the body or cells of another organism. Example of endosymbiosis is nitrogen-fixing bacteria or rhizobia that live in root nodules on legume roots. Another example is single-celled algae inside reef-building corals.

Random facts
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Muddiest point
  • Why is the bacteria in our body called normal flora? Because to me flora means plants.
My own exploration
Reflection on this topic

Today our group discussed on how to study well in the topic of protozoa. My members have different ideas on the best way to study, but I personally think that we need to visualize what we learnt. So I suggested them to choose watching videos and understanding each term as our learning method. In fact some of their ideas are helpful too like drawing mind map but I still think that somehow we need some animations to reinforce our understanding. On the other hand, the method done by Choy's group, using acronyms and songs, were really creative and helpful. It's very easy to remember all the facts.

Not related to the learning outcome again. So today was my birthday and I didn't expect that my university friends will celebrate with me because I thought most of them didn't know about it. At last, many surprises made by them and I'd received many blessings from them. I felt really happy and warm, thank you guys !

Lesson 8 (7/10/14)

Date : 7/10/14
Topic : Internal structures of bacteria (inclusions)

Activities
1. Quiz by using socrative

Notes

Name of microbes


Azotobacter is a genus of oval or spherical bacteria. They are motile and can form thick-walled cysts and may produce large quantities of capsular slime as well.They are aerobic, free-living soil microbes and can fix nitrogen into ammonium. It is used to produce biofertilizers, food additives and some biopolymers.
(E.g. Azotobacter vinelandii)

Halothiobacillus neapolitanus is a genus of proteobacteria, also classified with purple sulfur bacteria. All species are obligate aerobic bacteria. They have carboxysomes.

Aquaspirillum magnetotacticum is one of the four magnetotactic bacterium under gram-negative, microaerophillic (grow in low concentrations of oxygen) genus of magnetospirillum. They are spirillar or helical and have polar flagellum at each end. They live in shallow fresh water and sediments. They are magnetotaxis, meaning can orient themselves according to Earth's magnetic field and are aerotaxis, meaning will remain themselves in favourable oxygen concentration. When the bacteria ingest iron, proteins inside their bodies interact with iron to produce magnetite, Fe3O4.


Proteobacteria are major group of Gram-negative bacteria that includes purple bacteria, pathogens, free-living (non-parasitic) and nitrogen-fixing bacteria. Some have flagella, some are nonmotile or rely on bacterial gliding and some can aggregate to form multicellular fruiting bodies. Some are facultatively or obligately anaerobic, chemoautotrophs, and heterotrophic.

E.g. Escherichia, Salmonella, Vibrio, Helicobacter, Yersinia

Neocallimastix (-mastix = whips = many flagella) is a genus of obligately anaerobic rumen fungi. Besides fungi, microbial population of rumen also consists of bacteria and protozoan. They are lack of mitochondira, instead they use hydrogenosomes to oxideze NADH to NAD+ and release H2 as product. They can produce polysaccharide degrading enzymes to help in digestion of fibres.
Clostridium tetani is a box-car shaped, anaerobic Clostridium bacterium. They are Gram-positive and found as spores in soil or in gastrointestinal tract of animals. They produce tetanospasmin (potent biological toxin) that causes tetanus.

Clostridium perfringens is a Gram-positive, rod-shaped, anaerobic, spore-forming Clostridium bacterium. They can be found in nature as normal component of decaying vegetation, marine sediment, animals (especially in the intestinal tracts), insects and soil. They can cause food poisoning and infection such as necrosis, bacteremia (presence of bacteria in blood = abnomal because blood is sterile), emphysematous cholecystitis (infection of gallbladder) and gas gangrene.

Terms in microbiology

Volutin granules are an intracytoplasmic storage form of complexed inorganic polyphosphate.

Random facts
  • Microbial nutrition can be deduced from two factors, which are source of carbon and source of energy.
    • if source of carbon is CO2, and source of energy is light, then it is a photosynthetic microbe
    • if source of carbon is organic molecules, then it is a heterotroph
  • Hydrogen peroxide is also an ingredient in hair dye. Some microbes can avoid accumulation of hydrogen peroxide by having magnetosomes to avoid it. Some can neutralise the toxicit of hydrogen peroxide by secreting enzyme peroxidase. 
  • Gas vacuoles are normally found in protozoa and eukaryotes. Gas vesicles are normally forund in bacteria.
  • There are many types of sulfur bacteria : purple sulfur, green sulfur, non sulfur
  • There are many classes of proteobacteria : Alpha/Beta/Gamma/Delta/Epsilonproteobacteria
  • Endospores are very tough and resistant to harm. They can only be destroyed by using certain radiation.
  • Dipicolinic acid that is found in core wall of endospores is important to protect DNA against damage due to wet and dry heat, desiccation and genotoxic chemical.
  • Current topics in microbiology can be found in sciencedaily.com and microbial world.
  • Gas in gas gangrene is found to has the same composition as with atmospheric air.
Muddiest point
-

My own exploration
  • Teeming life in a drop of water
  • Foldscope
  • Nanoscope
  • http://www.microscopyu.com/moviegallery/pondscum/metopus/
  • http://en.wikipedia.org/wiki/Necrotizing_fasciitis
  • http://www.sciencelearn.org.nz/Contexts/Exploring-with-Microscopes/Sci-Media/Interactives/Which-microscope
  • http://en.wikipedia.org/wiki/HeLa
  • http://www.youtube.com/watch?v=8SuJxsQRnfw
  • http://www.youtube.com/watch?v=dRNYWX4v0VE
  • https://www.google.com.my/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&ved=0CD4QFjAG&url=http%3A%2F%2Fio9.com%2F5908318%2F10-surprising-things-that-bacteria-like-to-eat&ei=J0M2VOHOGtO7uASbjoDYDQ&usg=AFQjCNHJpCRpNl5pfnKge215KRGP5eGp0Q&sig2=Dn5z97qoeA5R32Af6t6Xtw&bvm=bv.76943099,d.c2E&cad=rja
Reflection on this topic


It's basically learning new information and there was no much reflection done in this topic. However, realizing that bacteria such as Clostridium bacteria can cause severe infection like gangrene and necrosis did terrify me. It made me realize the importance of good hygiene because indeed, prevention is better than cure.

Friday, October 3, 2014

Lesson 7 (3/10/14)

Date : 3/10/14
Topic : External structures of bacteria (cell wall) and internal structures of bacteria (plasma membrane, cytoplasm, nucleoid, plasmid, ribosomes)

Activities
1. Test on the spot : filling in the blanks for structure of bacteria and their respective roles

Notes

Species of microbes
  • Chlamydiaceae / Chlamydia / Chlamydophila (an obligate intracellular parasite)
    • similar to G-, has two membranes, the cell wall contains an outer lipopolysaccharide membrane but lacks of peptidoglycan
    • life-cycle alternates between non-replicating, infectious elementary body (analogous to spores) and replicating, non-infectious reticulate body
Terms in microbiology
  • Exoenzymes (secreted by G+ bacteria to degrade large nutrients)
  • Periplasmic space (area between inner/plasma membrane and outer membrane ; there is no or ve)
  • Teichoic acid (polysaccharide of glycerol phosphate or bibitol phosphate)
    • only found in G+ bacteria
    • negatively charged
    • confer acidity of cell wall
    • provide antigenic specificity ; pathogenicity based on teichoic acid
    • protect the cell wall from degrading easily
    • not all bacteira with teichoic acid is pathogenic
    • classes of teichoic acid
      • Lipoteichoic acid = teichoic acid that is attached to plasma membrane
      • Wall teichoic acid = teichoic acid that is linked to the peptidoglycan layer
  • Outer membrane in G- bacteria 
    • consists of lipoproteins, lipopolysaccharides, phospholipids and porins
    • provide a barrier against phagocytosis (has strong negative charge), certain antibiotic like penicillin, lysozyme, detergent, heavy metals, bile salts and certain dyes
    • due to minimal amount of peptidoglycan, G- bacteria is more vulnerable to mechanical breakage such as heating / cold / centrifuge / autoclave.
  • Lipopolysaccharide
    • lipo = Lipid A
      • as endotoxin (toxin that is produced inside the bacteria ; to be affected by the toxin, must ingest the bacteria and digest the cell wall to release the toxin ; thus the infection takes longer time to set it ; exotoxin can spread easily without breaking the bacteria's structure, thus is more dangerous)
      • when in host's bloodstream or gastrointestinal tract, the toxin causes fever or shock
    • polysaccharide = O polysaccharide
      • as antigens
      • to distinguish species of G- bacteria
  • Microbes with atypical cell walls (bacteria like mycoplasma and chlamydiaceae, archaea)
    • contains lipids called sterols that impart rigidity to the membrane and protect them from osmotic lysis (very similar to cholesterol)
  • ATP-binding cassette - ABC Transporters (consist of 2 hydrophobic membrane spanning domains, 2 cytoplasmic associated ATP-binding domains, substrate binding domains)
  • Group translocation (only found in prokaryotes ; substance is chemically altered during its transport across a membrane, once inside, the cytoplasmic membrane becomes impermeable to the substance so it remains inside the cell)
  • Protein domain (part of a protein's structure that can fold independently of the remainder of the protein)
  • Histone (any of a group of proteins found in chromatin ; cannot be found in bacterial chromosome)
Random facts
  • Difference between Mycoplasma and Mycobacteria
    • Mycoplasma = genus of bacteria that lacks a cell wall
    • Mycobacteria = acid-fast Gram positive bacteria that have a very thick, protective, waxy cell wall
  • Roles of cell wall:
    • Cell wall contributes to pathogenicity in some bacteria due to the presence of teichoic acid which contributes to acidity of cell wall as well. Some antibiotics act on cell wall to treat diseases.
    • Cell wall is also an important structure that provides nutrient to bacteria. \
    • Cell wall provides a rigid platform for surface appendages, such as flagella, pili and cilia that emanate from the wall and extend beyond it.
    • Cell wall is important to analyse and differentiate bacteria because cell wall of all bacterial are not identical.
  • The virulence (degree of pathogenicity) of bacteria depends on the species of bacteria rather than looking at the structure. Even though most G+ bacteria gain virulence from teichoic acid and most G- bacteria gain virulence from the outer membrane (especially lipopolysaccharides), there are still some exceptions.
  • Structure of archaea 
    • naturally resistant to lysozyme, penicillin, osmotic stress, pH, enzymes
    • cell walls of some archaea contain polysaccharide, glycoprotein, protein, pseudopeptidoglycan/pseudomurein
      • Example : paracrystalline surface layer (S-layer) which is made of protein or glycoprotein with hexagonal symmetry
        • S-layer is mainly found on archaea, just a few in G+ and G- bacteria. S-layer is also known as lipidless membrane. It involves in protection (against osmotic stress, pH, enzymes and phagocytocis) and may aid in attachment
  • Archaea were first found in extreme environments such as volcanic hot springs and salt lakes. Later, it was found that archaea live in a broad range of habitats like soils, oceans, marshlands, human colon and navel.
  • Plasma membrane also involves in 
    • synthesizing cell wall components
    • assisting DNA replication
    • carrying on respiration (oxidation and reduction ; aerobic and anaerobic)
    • capturing energy as ATP
  • For ribosomes, the total Svedberg unit is smaller than the sum of all subunits because when the subunits combine, they experience inevitably loss of surface area, which decreases the Svedbery unit because the sedimentation of centrifugation depends on the particle size and density.
Muddiest point
  • What is LOS?

My own exploration
  • In the name Escherichia coli O157:H7, the O refers the cell wall (somatic) antigen number, whereas the H refers flagella antigen. Other than that, K is also used for capsular antigens.
  • Rotavirus 
  • http://faculty.ksu.edu.sa/5115/Documents/Bacteria.pdf
  • http://discovermagazine.com/2006/dec/cover
  • http://www.eurekalert.org/pub_releases/2007-09/sfgm-bsi082807.php
  • http://www.hindawi.com/journals/jt/2012/862764/
  • http://www.cram.com/flashcards/bacteria-chlamydiaceae-mycoplasmas-and-anaerobic-bacteria-1282898
  • https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/bacteria-archaea-and-eukaryote-cell-structure-4/cell-walls-of-prokaryotes-34/cell-walls-of-archaea-263-284/
Reflection on this topic


For this lesson, I managed to revise on it before entering the class, even though I actually burnt the midnight oil the day before it. I found that I can understand the lecture easier and able to relate better with the information given during the lecture. By doing so, I had more fun learning under less stressful atmosphere throughout the lecture. That's the benefit of making early preparation.